Figure 3. GPVI-dependent ROS production and FAK activation are α_IIbβ₃-independent.

A. Washed platelets (1×10⁹/mL) were pre-incubated with RGD peptide (150 µg/mL) and then stimulated with 1 µg/mL CRP for 3 min (with stirring), lysed, immunoprecipitated with anti-FAK (4 µg), analyzed by SDS 5–20% polyacrylamide gel electrophoresis, and immunoblotted for phosphotyrosine with 4G10 or for FAK. Blots are representative of three independent experiments. IB, immunoblot. B–C. Washed platelets (2.5×10⁸/mL) pre-treated with various concentrations of RGD peptide (50–150 µg/mL) were stimulated with 1 µg/mL CRP and monitored for (B) platelet aggregation and (C) ROS generation. Aggregation traces are representative of three independent experiments.