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. 2014 Nov 21;9(11):e113333. doi: 10.1371/journal.pone.0113333

Figure 4. Analysis of expression of the genes encoding S100 proteins in SGs.

Figure 4

A: Microarray analysis of the genes encoding S100 proteins. Total RNA was prepared from SMGs from Myd88 +/+ mice and Myd88 -/- mice at 10 weeks old (n = 3 each). The mean value of fold-change for each gene in SMGs from Myd88 -/- mice shown here was calculated relative to expression of that gene in SMGs from Myd88 +/+ mice. Statistical analysis was performed by unpaired Student's t-test. *, P<0.05. B and C: qRT-PCR analysis for expression of S100a8 (left) and S100a9 (right) in SMGs (B) and SLGs (C). Total RNA was prepared separately from SMGs and SLGs from Myd88 +/+ mice and Myd88 -/- mice at 10 weeks old (n = 8 each). Expression levels of S100a8 and S100a9 in SMGs and SLGs were calculated relative to expression of the Hprt1 housekeeping gene. Means of each group were shown and P values were calculated by unpaired Student's t-test. *, P<0.05; **, P<0.01.