Figure 4. Human monocyte-derived macrophages and dendritic cells express integrin α_Dβ₂ during differentiation in culture.

Unfractionated monocytes were separated from the venous blood of healthy subjects by positive selection and differentiated to (A) macrophages or (B) monocyte-derived dendritic cells using procedures and protocols outlined in Materials and Methods. MDM and MDDC were examined for expression of α_Dβ₂ by flow cytometry on days 3, 6, and 8 in culture. These results are representative of findings in three experiments using cells from different subjects.