Table 1. Integrin αDβ2 is Dynamically Expressed by Human Monocyte-derived Macrophages (MDM) and Dendritic Cells (MDDC).
Experiment 1: MDM | |
Agonist | αD (MFI; % positive cells indicated in parentheses) |
Control | 88 (90) |
LPS | 40 (95) |
IL-4 | 97 (87) |
IFNg | 83 (84) |
Isolated human monocytes were cultured in human serum (Experiment 1) or M-CSF (Experiment 2) for 3 days to induce differentiation into monocyte-derived macrophages [28],[29]. After 6 days in culture they were treated with LPS, with IFNg as a stimulus for “classic activation”, or with IL-4 as a stimulus for “alternative activation” [46],[47]. In a third experiment monocytes were cultured in GM-CSF and IL-4 to induce differentiation to immature MDDC (“control”); they were then treated with LPS to trigger transition to mature MDDC (“LPS”) [29]. Results are expressed as mean fluorescence intensity (MFI), with the percentage of positive cells in parentheses.