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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Transfusion. 2014 May 21;54(12):3208–3215. doi: 10.1111/trf.12717

Figure 1. A soluble recombinant (sr) construct comprising extracellular Loop1 of CTL2 is recognized only by Type 1 HNA-3a antibodies.

Figure 1

Sr Loop 1 constructs containing R154 (black bars) or Q154 (gray bars) were immobilized in microtiter plates and their reactions with HNA-3a antibodies were characterized by ELISA. Only Type 1 antibodies reacted with the R154 construct. No antibodies recognized the Q154 construct. Studies were done in triplicate. Error bars represent ± 1.0 SD; *** = p<0.001 relative to the reaction with the Q154 construct. HD=Healthy donor serum. A Western blot performed with anti-myc (mAb 9E10) showed that equal quantities of the two constructs were tested (insert).