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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Transfusion. 2014 May 21;54(12):3208–3215. doi: 10.1111/trf.12717

Figure 4. Recombinant mouse CTL2 (mCTL2) is recognized only by Type 1 HNA-3a antibodies.

Figure 4

HEK293 cells expressing mCLT2 were sensitized with the HNA-3a antibody panel and washed. Bound human antibody was detected by flow cytometry. Values on the ordinate indicate ratio of median fluorescence intensity (MFI) obtained with cells expressing mouse CTL2 to that obtained with cells expressing human CTL2 (Q154) (not shown). Mouse CTL2 was recognized only by the Type 1 HNA-3a antibodies 1 and 5. Studies were done in triplicate. Error bars represent ± 1.0 SD, ** = p<0.01 relative to reaction with human CTL2 Q154. HD=Healthy donor serum.