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Translation of the proteins CoxD, CoxE and CoxF in cell-free crude extracts of wild-type O. carboxidovorans and its insertional mutants D::km, E::km or F::km. Bacteria were cultivated under chemolithoautotrophic conditions with a gas atmosphere composed of (v/v) 30 % H2, 5 % CO2, 30 % CO, and 35 % air. Cell-free crude extracts (200 µg protein/lane) were subjected to denaturing PAGE followed by Western blotting employing IgG antibodies directed against CoxD (a) CoxE (b) or CoxF (c)
