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UV/VIS absorption spectra of CO dehydrogenases purified from wild type (a) or from the mutants in E::km (b), F::km (c) and G::km (d). Traces: a, air-oxidized; b, sparged with pure CO for 30 min; c, reduced with 650 µM dithionite under N2 for 4 min. The insets show the visible part of the spectra at greater detail
