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CO dehydrogenases from the mutants in coxE (a) or coxF (b) were treated with Cu1+-(thiourea)3 (filled circle) or with sodium sulfide and sodium dithionite first followed by Cu1+-(thiourea)3 (open circle). For the removal of Cu and cyanolyzable sulfur, CO dehydrogenases were incubated with potassium cyanide and then treated with sulfide/dithionite and copper as above (filled triangle). For details see the “Methods” section
