Table 1.

Effect of E7080 on cell viability of human CRC cells and human umbilical vein endothelial cells (HUVEC).

IC₅₀ values were determined following E7080 treatment for 72 hours in media with low serum (1% (v/v) FCS). Cell lines are arranged according to increasing IC₅₀ values. Shown are the results as mean and 95% confidence interval (CI) of IC₅₀ of at least three independent proliferation assays with hexaplicates. IC₅₀ values and 95% CI were determined using GraphPad Prism software. See also Fig. 1A.Wild-type (wt) KRAS and mutated (mut) KRAS were determined with Sanger Catalogue of somatic mutations in cancer (COSMIC) database.

(http://cancer.sanger.ac.uk/cancergenome/projects/cosmic/).

Cell lines	KRAS status	IC50 (μmol/l)	95% CI (μmol/l)
CaCo2	wt	> 1000	¯¯¯¯
Colo741	wt	> 1000	¯¯¯¯
SW620	mut	548	273-111
HT29	mut	431	299-620
HCT116	mut	399	296-538
T84	mut	276	179-427
SW480	mut	240	183-313
Colo678	wt	222	175-283
LS174T	mut	144	96-215
HCT15	mut	120	91-158
HUVEC	wt	22	14-36