Figure 2.

CNBs induce an immunosuppressive tumor microenvironment. (A) Mice were implanted with 4T1 cells, and after 6- to 8-mm diameter tumors developed, half the mice were biopsied (n = 30), while the other half were not biopsied (n = 30). The tumor, spleen, and lungs were collected from 10 mice from each group for flow cytometry analysis of the indicated immune cells at 3, 7, and 10 days after the biopsy date. NK cells were only monitored on days 3 and 7 post-biopsy date. Significance determined by unpaired t test, *P ≤ .05; error bars represent SEM.