Figure 3.
CNB modulates the expression of pro-inflammatory factors within the local tumor microenvironment. Tumor samples collected from biopsied and non-biopsied mice were processed, and RNA was extracted, purified, and reversed transcribed using random primers. Real-time qPCR was conducted on the following gene-specific primers: (A) S100A8, (B) CXCL2 (MIP-2α), (C) CCL3(MIP-1α), (D) COX2, (E) IL-1β, and (F) TNF-α. Gene expression at 0, 3, 12, and 24 hours post biopsy was analyzed using ΔΔCt analysis with relative normalization with murine GAPDH and β-actin (n = 5; significance is determined relative to levels at 0 hour post biopsy by unpaired t test, *P ≤ .05; error bars represent SEM).