Figure 1.

FOXM1 is upregulated by E6, not by E7, in HPV-infected cancer cells. (A) E6, E7, FOXM1, and MMP2 protein expressions were evaluated by Western blot analysis in a panel of cancer cells with or without HPV infection. (B) E6 and E7 expressions were depleted by shRNA in SiHa, GNM, and TL-1 cells. p53, E6, E7, E2F1, Rb, pRb, and FOXM1 were detected by immunoblot analysis with antibodies. These lysates were immunoprecipitated with anti–Rb-conjugated beads. The immunoprecipitates were analyzed through SDS-PAGE, followed by immunoblot analysis with anti-E2F1 antibody. Quantitative real-time PCR amplification of FOXM1 was performed using total cellular RNA extracts. β-Actin was used as a loading control for the whole-cell lysates. Values are given as means ± SEMs for triplicate samples.