Figure 6.

Klf5 deletion upregulates EGF in Pten-null mouse prostate tumors and human prostate epithelial cell line. (A, B, and E) Detection of EGF mRNA expression by real-time RT-PCR (A) and Egf and p-Egfr proteins by IHC staining (B and E) in 6-month-old Pten-null mouse DPs with indicated Klf5 deletion status. Data for each genotype in A was from eight mice. (C and D) Detection of protein expression of KLF5 and PTEN by Western blot analysis (C) and mRNA expression of KLF5, PTEN, and EGF by real-time RT-PCR (D) in the PNT2 immortalized human prostate luminal epithelial cell line with the knockdown of KLF5, PTEN, or both by RNA interference.