Figure 7.

Molecular characterization of prostate tumors induced by Klf5 and Pten deletion. (A) IHC staining of the Ki67 proliferation marker, oncogenic kinases p-Akt, p-Erk1/2, and p-S6, and the p15 cell cycle inhibitor in tissue sections of APs at 6 months of age with different deletion status of Klf5 and Pten. Marker names are indicated at the left, and the deletion status of genes is indicated at the top. The ratio (%) of Ki67-positive cells, as determined by cell counting, is shown at the lower right corner of each image of the Ki67 panels. (B) Detection of protein expression for Klf5, p-Akt, Akt, p-Erk1/2, Erk1/2, p-S6, p15, and β-actin by Western blot analysis in tissue lysates from anterior mouse prostates with the same genotype and age used in A. Relative protein level was determined by using the ImageJ software, and statistical significance was evaluated by using Student’s t test. Marker names are indicated at the left, and the deletion status of genes is indicated at the top. + and − indicate the presence and absence of an allele for Klf5 and Pten. *P < .05; **P < .01.