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. Author manuscript; available in PMC: 2015 Apr 30.
Published in final edited form as: Nat Commun. 2014 Oct 31;5:5322. doi: 10.1038/ncomms6322

Figure 2.

Figure 2

Active site of Osa is essential for T-DNA transfer in vivo. Results of GUS (β-glucuronidase) assay after agroinfiltration of Nicotiana tabaccum leaf discs are shown. Blue-green color indicates GUS expression, and thus transfer of gus gene flanked with T-DNA borders from Agrobacterium into plant. Blank: Agrobacterium resuspension buffer, Mock: Agrobacterium tumefaciens LBA4404 strain alone, Control: LBA4404 containing pCAMBIA1301 and pSoup vector alone, without any insert. Test experiments had Agrobacterium clones harboring pCAMBIA1301 and pSoup containing either Osa or one of its mutants, or other FINs; ICE1056Fin, FipA and PifC.