Figure 4.

Schematic representation of a case of conflict (ltc ≥ 2fpc) in fingerprinted contigs (fpc) versus linear topological contigs (ltc), as a reference. Anchored genetic markers (M) were used to confirm the corresponding bacterial artificial chromosome (BAC) contig structure. Three situations were observed. Status 1: concordant M₁ and M₂ genetic positions, with the genetic position for the corresponding marker in at least one fpc contradictory; ltc 001 correctly assembled. Status 2: non-concordant M₁ and M₂ genetic positions, with the genetic position of the corresponding marker in both fpc contigs reconciled; ltc 001 not correctly assembled. Status 3: concordant M₁ and M₂ genetic positions, with the genetic position for the corresponding marker in both fpc contigs reconciled; all contigs correctly assembled. Red/blue bars, BAC clones underlying BAC contigs; dashed bars, connect shared BACs; green arrowheads, genetic marker position; red arrowheads, region of low BAC coverage.