Table 2.
Overview of general whole-genome profiling (WGP™) parameters and sequence data processing
| Parameter | 6AS | 6AL |
|---|---|---|
| Estimated size of chromosome | 336 Mbp | 369 Mbp |
| WGP™ tag length, incl. restriction sitea | 50 nt | 50 nt |
| No. of BACs tested | 22 656 | 24 576 |
| Chromosome arm equivalent BACs tested | 8.4 | 7.0 |
| No. of high-quality reads produced (M) | 99.8 | 165.4 |
| No. of deconvolutable reads (M) | 52.2 | 78.2 |
| Percentage of deconvolutable reads (%) | 52.30 | 47.30 |
| No. of tagged BACs | 19 289 | 18 660 |
| No. of tagged BACs used in assembly | 18 820 | 17 309 |
| No. of unique WGP™ tags | 109 611 | 108 811 |
| No. of unique WGP™ tags used in assembly | 109 570 | 108 700 |
| Percentage of tagged BACs (%) | 85 | 75.9 |
| Average no. of WGP™ tags/BAC | 29.7 | 28.6 |
| Average no. of reads/tag | 61.4 | 111.1 |
BAC, bacterial artificial chromosome.
b
The enzyme combination applied was HindIII/MseI for both libraries.