Table 3.

Cofilin-Actin Binding Data as Determined by Quenching of Pyrene Fluorescence.

Cofilin:actin	t (s)	WT cofilin	Cof1-157p	Cof1-158p	Cof1-159p
4:1	50	ND	ND	0.91±0.04	ND
	300	ND	ND	0.94±0.04	ND
3:1	50	ND	ND	0.86±0.07	0.92±0.06
	300	ND	ND	0.94±0.06	0.94±0.06
2:1	50	0.90±0.02	0.93±0.01	0.67±0.15	0.94±0.04
	300	0.92±0.02	1.00±0.004	0.90±0.08	1.00±0.04
1:1	50	0.97±0.02	0.87±0.02	0.22±0.06	0.44±0.04
	300	0.96±0.02	0.87±0.03	0.41±0.05	0.74±0.02
2:3	50	0.79±0.04	0.57±0.02	0.18±0.03	0.23±0.02
	300	0.74±0.04	0.61±0.02	0.31±0.03	0.45±0.02
1:2	50	0.66±0.03	0.40±0.02	0.12±0.07	0.16±0.09
	300	0.62±0.03	0.43±0.02	0.22±0.06	0.33±0.07
1:4	50	0.30±0.01	0.12±0.02	0.05±0.02	0.10±0.03
	300	0.28±0.01	0.18±0.02	0.11±0.02	0.19±0.02
1:6	50	0.13±0.05	0.04±0.03	ND	NA
	300	0.13±0.02	0.07±0.04	ND	0.06±0.10
1:8	50	0.13±0.04	0.03±0.04	ND	ND
	300	0.13±0.02	0.07±0.04	ND	ND

Pyrene quenching of 2 μM actin filaments is expressed as a percentile fraction of the maximal quenching observed 50 and 300 seconds after cofilin addition. The values reported are the averages of three experiments.