Figure 5.

Effects of emodin on PKCδ activation in aortic rings without endothelium. (A) Aortic rings without endothelium were pretreated with emodin for for 2 h, PDBu-induced contraction was then measured. (B) Aortic rings without endothelium were pretreated with emodin (10 μM) for 2 h, aortic rings were stimulated with PDBu (10⁻¹⁰ M) for 2 min and protein was extracted from the aortic tissues. CPI-17 phosphorylation was determined by western blotting. (C, D) Aortic rings without endothelium were treated with emodin (10 μM) for 2 h, they were then stimulated with phenylephrine (10⁻⁵ M) for 2 min, and protein was extracted from aortic tissues. (C) PKCα and (D) PKCδ activation was determined by PKC phosphorylation through Western blotting. Values are mean ± SEM of three to four independent experiments. *Represents significant differences from control (anova and Duncan's post hoc analysis, P < 0.05).