Figure 2.
H2S increases glycolysis in cancer cells. An increase in (A) glucose uptake and (B) glucose consumption was observed in MCF7 cancer cells incubated with GYY4137. Antimycin A (AA, 2 μM) was included as a positive control. LSC, liquid scintillation count. (C) Significantly higher glycolytic enzymes reaction rate in both GYY4137-treated HepG2 (left) and MCF7 (right) cancer cells. HK, hexokinase; PFK, phosphofructokinase; PK, pyruvate kinase. (D) A large increase of lactate amount and (E) enhanced lactate production rate in both MCF7 and HepG2 cancer cells upon H2S incubation. NT, non-treated. ZYJ1122 and GYY4137, 400 μM. (n = 3), *P < 0.05. Results are mean ± SD.