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A: Construction of the expression vector for pMistic-MPR-TMTEV-6His. See text and Supporting Information for details. B: Scheme of the Mistic-MPR-TMTEV-6His fusion protein. C: Amino acid sequence of Mistic-MPR-TMTEV-6His. Purple: His-tag; Green: Mistic; red: MPR-TM; underlined ELDKWA: the mAb 2F5 core epitope; underlined NWFDI: the 4E10 mAb core epitope; blue: TEV recognition sites. Note that the cleavage occurred between Q and G residues of the TEV recognition sequence ENLYFQG.
