Figure 10.

Purified native MPR-TM_TEV-6His can bind to 2F5 and 4E10 mAbs. A: Image of the developed ELISA plate. Row A: control uncoated wells. Rows B-D: wells coated with serially diluted MPR-TM_TEV-6His. Rows E, F, and H: wells coated with serially-diluted CTB-MPR. Row G: wells coated with serially-diluted CTB. All rows except B and H were overlaid with the mAb 2F5. (B, C) SPR analysis. Association/dissociation traces of MPR-TM_TEV-6His (B) and CTB-MPR (C) with either 2F5 or 4E10 mAbs. Traces are average of four independent measurements and the dissociation constants (K_D) are listed in the inserts (mean ± SD).