Table 2.
B-Factor Values (Å2)a of Phe34 in Two bGAPDH Structures
| Subunits | bGAPDH(NAD)3 (Å2) | bGAPDH(NAD)4 (Å2) | Difference (Å2) |
|---|---|---|---|
| O | 45.1 (NAD free) | 13.3 (+8.4) | −23.4 |
| P | 36.5 | 16.3 (+8.4) | −11.8 |
| Q | 28.6 | 16.6 (+8.4) | −3.6 |
| R | 33.0 | 14.6 (+8.4) | −10 |
| Average for whole protein | 26.1 | 17.7 | −8.4 |
a
The difference in overall average B-factor value was 8.4 between the two structures. To compare the B-factor values of Phe34 from the two structures, a constant value of 8.4 was added to the B-factor value for Phe34 in bGAPDH(NAD)3 that had the lower B-factor value.