Table 1.
Mpdz knockdown in NS20Y cells. Five Mpdz shRNA plasmids were assessed for relative knockdown of Mpdz expression in NS20Y cells compared to scrambled control using QPCR. Each hairpin sequence is comprised of a 21 base stem (with the 21 underlined bases indicating the bases that hybridize to the Mpdz sequence in Exon 10) and a 6 base loop.
| Plasmid | Targeted Sequence | Mpdz expression |
|---|---|---|
| TRCN0000103480 | CCGGCGCACCAGAATGAGTGTGTTTCTCGAGAAACACACTCATTCTGGTGCGTTTTTG | 1.05 |
| TRCN0000103481 | CCGGGCTCTGATAGATACACCTGATCTCGAGATCAGGTGTATCTATCAGAGCTTTTTG | 0.24 |
| TRCN0000103482 | CCGGGCCTTCAGGAATCTTTGTAAACTCGAGTTTACAAAGATTCCTGAAGGCTTTTTG | 0.23 |
| TRCN0000103483 | CCGGGCATCTGAAATTCAGGGACTACTCGAGTAGTCCCTGAATTTCAGATGCTTTTTG | 0.62 |
| TRCN0000103484 | CCGGCATCTGAAATTCAGGGACTAACTCGAGTTAGTCCCTGAATTTCAGATGTTTTTG | 0.50 |
| SCH002 | CCGGCAACAAGATGAAGAGCACCAACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTT | 1.00 |