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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Genes Brain Behav. 2014 Sep 29;13(8):743–757. doi: 10.1111/gbb.12175

Figure 4. Verification of Differential Expression of Gnb1 in HAP3 vs LAP3 Mice by qRT-PCR.

Figure 4

RNA was isolated from brains of three individual HAP3 and LAP3 mice (generation 12). qRT-PCR was performed on the Roche LightCycler 480 using primers and probes from ABI. Samples from all mice were assayed in triplicate in the same assay. Data were corrected for two endogenous controls (Gapdh and Pkg1), and relative quantities (ratio of target to standard, mean ± SEM) were calculated using the LightCycler 480 software. *P<0.006 (Student’s t-test).