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. 2014 Nov 18;14:228. doi: 10.1186/s12862-014-0228-6

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© Sveinsson and Cronk; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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A maximum likelihood phylogeny of Trifolium . Phylogenetic relationships among the Trifolium species, generated from a concatenated matrix of 58 protein coding plastid genes with a combined aligned length of 48,058 characters. The black dot marks the clade with plastomes refractory to assembly and the black vertical lines indicate the plastome size categories.The age of the most recent common ancestor (MRCA) of the refractory clade is estimated to be 12.4 – 13.8 million years old. All nodes have a 100% bootstrap support.