Fig. 4.

Biochemical investigation of SR-BI localization in vivo in rat liver. a Western blot analysis of starting material, intermediate aliquots and final canalicular membranes is shown. S1 supernatant 1; S2 supernatant 2; SA supernatant A; SB supernatant B; SC supernatant C; CM canalicular membrane. b Enrichment of corresponding proteins was assessed by densitometric calculation (protein) and measured enzymatic activity (enzyme). Note the enrichment of SR-BI in membrane fractions of rat liver representing canalicular membranes