Table 2.

Conventional RT-PCR and QPCR analyses of Ccne1—cyclin E, Ccnd1—cyclin D1, Ccnb1—cyclin B, Nek6—NIMA- (never in mitosis gene a-) related kinase 6, Pttg1—pituitary tumor-transforming 1, Cdk4—cyklin-dependent kinase 4, Cdk6—cyklin-dependent kinase 6, Cdk1—cyklin-dependent kinase 1, Cdkn1a—cyclin-dependent kinase inhibitor 1A, Cdkn1c—cyclin-dependent kinase inhibitor 1C, Egfr—epithelial growth factor receptor, Igf1r and Igf2r—insulin growth factor receptors 1 and 2, Mc2r—ACTH receptor, Fos—FBJ osteosarcoma oncogene, transcription factor and Junb—jun B proto-oncogene, transcription factor. Oligonucleotide sequences for sense (S) and antisense (A) primers are shown. Hprt (hypoxanthine phosphorybosyl transferase) was the reference gene.

cDNA	Genbank accession number	Primer	Primer sequence (5′-3′)	Position	PCR product size (bp)
Ccne1	NM_001100821.1	S	AAGACTGTGAAAAGCCAGGA	320–339	176
		A	AAGACGGGAAGTGGGGAGG	477–495
Ccnd1	NM_171992	S	AACAAGCAGATCATCCGCAAA	645–666	213
		A	GAGGCAGTCCGGGTCACA	840–858
Ccnb1	NM_171991.2	S	TTAAAGCCCTACCAAAACC	313–331	207
		A	CAAGAATCACATCGGAGAA	501–519
Nek6	NM_001277232.1	S	CAGCCCAGCCACATGCCTC	32–50	151
		A	CTCGGCCAATCTTCTTCTC	164–182
Pttg1	NM_022391.2	S	GTCTCTCCCCTCAGTAATCCA	258–278	171
		A	CACCGAACACTTTGCCGACT	409–428
Cdk4	NM_053593	S	CAAGTAATGGGACAGTTAAG	631–651	208
		A	GAGTTCCCACAGAAGAGAG	820–839
Cdk6	NM_001191861.1	S	AATAAAACTGGCTGACTTCGG	474–494	205
		A	ATCCACGTCTGAACTTCC	661–678
Cdk1	NM_019296.1	S	ACAGAGAGGGTCCGTTGT	67–85	175
		A	CGTACTGGGCACTCCTTCTT	222–242
Cdkn1a	NM_080782	S	CTTGTCGCTGTCTTGCACT	377–395	241
		A	CACTGAATGAAGGCTAAGG	599–618
Cdkn1c	NM_001033757	S	GCCTCTCTCGGGGATTCCA	1121–1140	240
		A	TCTAAACTAACTCATCGCAGAC	1339–1361
Egfr	NM_031507.1	S	ATTGCCCTGAACACCGTGGA	424–443	176
		A	CGCACAGCACCGATCAGAA	581–599
Igf1r	NM_052807.2	S	GACAGGAGTACAGGAAGTATGG	2693–2714	197
		A	AATCAGCAGGATGGCAACC	2871–2889
Igf2r	NM_012756.1	S	CATCTCTGTTCATCAATGTG	676–695	164
		A	CCTGTCCTTGCTCAATAG	822–839
Mc2r	NM_001100491.1	S	GGACAAGGGGGGAGGCAGA	100–119	201
		A	TGGCACAACTACATCAGGAC	281–301
Fos	NM_022197.2	S	TTTCAACGCGGACTACGAG	167–185	164
		A	AGTTGGCACTAGAGACGGAC	311–330
Junb	NM_021835.3	S	AATGGAACAGCCTTTCTATCA	282–302	99
		A	GGTTTCAGGAGTTTGTAGTC	361–380
Hprt	NM_012583	S	CAGTCAACGGGGGACATAAAAG	391–412	146
		A	ATTTTGGGGCTGTACTGCTTGA	515–536