Figure 4. Dose-dependent effects of nocodazole on EC permeability and microtubule arrangement.

A – EC were treated with the indicated concentrations of nocodazole, and changes in EC permeability were monitored by TER measurements. Shown are representative data from three independent measurements. B – Immunofluorescence staining of microtubule cytoskeleton in EC treated with various nocodazole concentrations was performed using β-tubulin antibody.