A-C) Liver section from a rat raised on a low-Cu diet for 38-45 days since birth. D-F) Liver section from a low copper rat given Cu then sacrificed after 60 minutes, or G-I) 120 minutes. Rat livers were fixed by immersion and cryosections were immunolabeled for ATP7B (green), TGN38 (red), or apical surface marker DPP4 (blue) then imaged by confocal microscopy. (G-I), Arrows indicate the transient appearance of ATP7B in the basolateral region 60 minutes after Cu injection. J) Quantification of Cu-induced trafficking in vivo. The graph depicts colocalization analysis on confocal stacks from liver sections stained above. Cu causes a decrease of ATP7B in the Golgi (solid line), with concomitant increase in ATP7B trafficking to the apical domain (dotted line).