Figure 1.

Site-specific ¹³C–¹H RDCs are measured using a 2D DROSS spectrum for the case of the POPC/cholesterol (1:1) binary mixture at 30°C. (a) Selected recoupled powder patterns showing experimental (gray) and simulated (red) lineshapes. (b) Oblique view of the aliphatic fingerprint region of the DROSS spectrum of the binary system POPC/cholesterol. (c) A 2D plane of the spectrum shown in b. The ¹³C isotropic chemical shift (δ) spectrum is shown along F₂ (the horizontal axis) (red). The peak separation of the Pake doublet yields the site-specific ¹³C-¹H dipolar coupling along F₁ (the vertical axis). Segmental |S_CH| order parameter values are calculated as a function of peak position according to Eq. 2. To see this figure in color, go online.