Skip to main content
. 2014 Nov 18;107(10):2456–2466. doi: 10.1016/j.bpj.2014.10.007

Figure 2.

Figure 2

The SNAP-tagged proteins are biologically active. (A) Stat1 phosphorylation in K562 CD20+ cells induced by SNAP-IFN fusion proteins after 30 min. Both IFNα(WT)-SNAP and IFNα(R144A mutant)-SNAP induced robust Stat1 phosphorylation in K562 CD20+ cells (the EC50 values were 0.04 nM and 17.13 nM, respectively). The decrease in IFNα activity due to the R144A mutation was consistent with previous reports (4,8). (B) Fluorescent staining of K562 WT and CD20+ cells with DAPI and anti-CD20 scFv-SNAP conjugated to Alexa Fluor 546. (C) Stat1 phosphorylation assay control performed in K562 CD20+ cells with the non-IFN components of the chimeric activator complex. (D) Gel-shift assay to demonstrate oligonucleotide conjugation to protein complexes via changes in electrophoretic mobility. To see this figure in color, go online.