Table 1.
Thickness and derived properties of the lipid bilayers with and without N1 and N2 peptide bound from the fitting of the neutron reflectometry data
| Layer | Thickness (no peptide) / Å | φlipid (no peptide) | Thickness (peptide bound) / Å | φlipid (peptide bound) | φpeptide | Γlipid / μmol m−2 | Γpeptide / μmol m−2 |
|---|---|---|---|---|---|---|---|
| d31-POPC/d31-POPG (2:1) bilayer + N1 peptide | |||||||
| Headgroup 1 | 15 ± 1 | 0.94 ± 0.02 | 18 ± 1 | 0.75 ± 0.01 | - | 7.46 ± 0.12 | - |
| Tails | 20 ± 1 | 1.00 ± 0.02 | 23 ± 1 | 1.00 ± 0.01 | - | 4.11 ± 0.01 | - |
| Headgroup 2 | 10 ± 1 | 0.41 ± 0.02 | 7 ± 1 | 0.10 ± 0.01 | 0.47 ± 0.01 | 0.39 ± 0.01 | 0.07 ± 0.01 |
| Peptide | - | - | 21 ± 2 | - | 0.15 ± 0.01 | - | 0.08 ± 0.01 |
| d31-POPC/d31-POPS (2:1) bilayer + N1 peptide | |||||||
| Headgroup 1 | 7 ± 1 | 0.94 ± 0.06 | 8 ± 1 | 0.99 ± 0.03 | - | 4.58 ± 0.16 | - |
| Tails | 20 ± 1 | 1.00 ± 0.02 | 29 ± 1 | 0.97 ± 0.01 | - | 4.85 ± 0.05 | - |
| Headgroup 2 | 11 ± 1 | 0.94 ± 0.03 | 9 ± 1 | 0.08 ± 0.01 | 0.47 ± 0.01 | 0.42 ± 0.01 | 0.07 ± 0.01 |
| Peptide | - | - | 21 ± 1 | - | 0.25 ± 0.01 | - | 0.08 ± 0.01 |
| d31-POPC/d31-POPG (2:1) bilayer + N2 peptide | |||||||
| Headgroup 1 | 7 ± 1 | 0.56 ± 0.01 | 7 ± 1 | 0.56 ± 0.02 | - | 2.30 ± 0.09 | - |
| Tails | 27 ± 1 | 1.00 ± 0.02 | 31 ± 1 | 1.00 ± 0.02 | - | 5.38 ± 0.12 | - |
| Headgroup 2 | 11 ± 1 | 0.86 ± 0.03 | 12 ± 1 | 0.14 ± 0.05 | 0.33 ± 0.01 | 0.90 ± 0.03 | 0.08 ± 0.01 |
| Peptide | - | - | 27 ± 3 | - | 0.20 ± 0.03 | - | 0.12 ± 0.01 |
| d31-POPC/d31-POPS (2:1) bilayer + N2 peptide | |||||||
| Headgroup 1 | 13 ± 1 | 0.89 ± 0.02 | 12 ± 1 | 0.78 ± 0.02 | - | 5.44 ± 0.11 | - |
| Tails | 26 ± 1 | 0.98 ± 0.01 | 27 ± 1 | 0.89 ± 0.01 | - | 4.17 ± 0.05 | - |
| Headgroup 2 | 18 ± 1 | 0.24 ± 0.02 | 16 ± 1 | 0.20 ± 0.01 | 0.53 ± 0.01 | 1.90 ± 0.03 | 0.18 ± 0.01 |
| Peptide | - | - | 20 ± 2 | 0 | 0.28 ± 0.01 | - | 0.12 ± 0.01 |