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. 2014 Nov 18;107(10):2245–2252. doi: 10.1016/j.bpj.2014.10.015

Figure 1.

Figure 1

(a) The modified double wall-ring flow cell. The Teflon flow cell is filled with media and the du Noüy ring is positioned at the air-liquid interface. (b) Schematic of the experimental setup. Media is added to the outer chamber to account for evaporative losses during the experiment. Excess media is aspirated from the outer chamber at a specific height to maintain the liquid level in both the inner and outer chambers throughout the experiment without disturbing the pellicle. (c) The elastic interfacial moduli of the rugose pellicle as a function of time measured using three different strain amplitudes. The reproducibility of the data confirms that the strain amplitudes used in this study do not perturb the formation of the pellicle. To see this figure in color, go online.