(a) Rugose, RΔrbmA, RΔbap1, and RΔrbmC pellicles growing in the Teflon flow cell over time. The images were captured without the du Noüy ring for easy visualization. (b) Evolution of the interfacial elastic moduli of the rugose, RΔrbmA, RΔbap1, and RΔrbmC pellicles. The rugose data is shown in the leftmost plot; subsequent plots compare the mutant data (black) to the rugose parent strain (gray). The data shown is representative of three trials. (c) Evolution of the interfacial elastic moduli of the RΔrbmAΔbap1, RΔrbmAΔrbmC, RΔbap1ΔrbmC, and RΔrbmAΔbap1ΔrbmC pellicles (black) referenced to the rugose pellicle (gray). The data shown is representative of three trials. To see this figure in color, go online.