Figure 2.

Histology-directed tissue layer profiling for matrix-assisted-laser desorption/ionization mass spectrometry. Digital photomicrographs acquired from histology and matrix-assisted-laser desorption/ionization sections were used to identify and designate sites of interest for profiling. Comparisons were performed in both the training and independent test set samples between inflamed mucosa Crohn’s colitis (CC) vs ulcerative colitis (UC) and inflamed submucosa CC vs UC. Tissue section showing marked areas of pathological interest. Rings demonstrate matrix spots in mucosal and sub-mucosal layers (unpublished figure).