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. Author manuscript; available in PMC: 2015 May 21.
Published in final edited form as: Nat Commun. 2014 Nov 21;5:5369. doi: 10.1038/ncomms6369

Figure 8. Repertoire of H5N1-rHA1 derived peptides appears the same with or without DM.

Figure 8

Samples containing equal starting materials (same amount of DR1 and rHA1 protein) were prepared in the presence, or absence of DM and the eluted peptides were analyzed by MALDI. (a–d) Native form of H5N1-rHA1 protein, DR1, and the cathepsins B, and H, and S were incubated in the (a) presence or (b) absence of DM. (c) and (d) are background spectra for samples a and b, respectively, from reactions not containing H5N1-rHA1. Immunodominant epitopes, HA(259–274) (SNGNFIAPEYAYKIVK), and HA(259–278) (SNGNFIAPEYAYKIVKKGDS), are shown at m/z 1814.7Da and 2200.8Da. The experiments were repeated three times.