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. 2014 Nov 24;4:7167. doi: 10.1038/srep07167

Figure 1. The expression of CS-wnt4a in C.semilaevis.

Figure 1

(A). The expression of CS-wnt4a in different tissues H: heart, L: liver, G: gill, S: skin, K: kidney, I: intestine, Br: brain, Sp: spleen, M: muscle, P: pituitary, O: ovary, T: testis. (B). The expression profile of CS-wnt4a at different developmental stages in the gonads was revealed by real-time quantitative PCR. 7D: 7 day age, 48D: 48 day age, 70D: 70 day age, 160D: 160 day age, 1Y: 1 year age, 2Y: 2 year age. (C) Low magnification showing the adult ovary architecture including four stages of oocytes by in situ hybridization: stage I–IV. (D) Large magnification of frame area in (C). (E) Low magnification showing the adult testis architecture including spermatogonaia (sg), primary spermatocytes (psc), secondary spermatocytes (ssc), spermatids (st), and sperm (sm) by in situ hybridization. (F) Large magnification of frame area in (E) highlighting the distribution of CS-wnt4a RNA.