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. 2014 Nov 24;4:7162. doi: 10.1038/srep07162

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HEp-2 and KB cells were treated with or without Mith for 48 h. (A) Cell numbers were obtained by direct cell counting with a hemocytometer. (B) By fluorescence microscopy (magnification X400), HEp-2 and KB cells exhibited nuclear fragmentation and condensation after DAPI staining. (C) HEp-2 and KB cells were treated with Mith as indicated for 48 h before western blots of whole cell lysates detected caspase 3, poly(ADP-ribose) polymerase (PARP) and cleavage of caspase 9. (D) HEp-2 and KB cells were preincubated with pancaspase inhibitor zVAD-fmk for 1 h before Mith treatment and western blot to detect cleaved PARP. β-actin was the internal control. Results are mean ± SD from three independent experiments. * P < 0.05.