Table 1.
Glycosyl composition of per-O-trimethylsilyl (TMS) derivatives of methyl glycosides performed on purified capsular polysaccharides from WT Caulobacter (NA1000), the single mutants ΔCCNA_00166 (ΔhvyA) and ΔCCNA_00163 and the ΔhvyA ΔCCNA_00163 double mutant
| NA1000 | ΔhvyA | ΔCCNA_00163 | ΔhvyA ΔCCNA_00163 | |||||
|---|---|---|---|---|---|---|---|---|
| Glycosyl residue | Mass (μg) | Weight (%) | Mass (μg) | Weight (%) | Mass (μg) | Weight (%) | Mass (μg) | Weight (%) |
| Ribose | 0.8 | 0.4 | 0.1 | 0.1 | 0.3 | 0.3 | 0.5 | 0.8 |
| Rhamnose | 2.3 | 1.2 | 1.1 | 0.6 | 6.4 | 6.2 | 2.7 | 4.3 |
| Fucose | 19.8 | 10.3 | 26.5 | 14.7 | 0.0 | 0.0 | 0.2 | 0.4 |
| Xylose | 0.0 | 0.0 | 0.1 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 |
| Glucuronic Acid | 0.0 | 0.0 | 0.0 | 0.0 | 0.8 | 0.8 | 0.3 | 0.5 |
| Galacturonic acid | 28.4 | 14.9 | 32.6 | 18.1 | 5.6 | 5.4 | 2.3 | 3.7 |
| Mannose | 23.2 | 12.1 | 26.6 | 14.8 | 3.9 | 3.8 | 1.7 | 2.7 |
| Galactose | 30.1 | 15.7 | 37.0 | 20.6 | 1.4 | 1.4 | 0.5 | 0.9 |
| Glucose | 64.3 | 33.6 | 52.9 | 29.4 | 49.9 | 48.5 | 23.7 | 38.3 |
| N-Acetyl galactosamine | 2.0 | 1.1 | 0.0 | 0.0 | 2.3 | 2.2 | 1.0 | 1.7 |
| N-Acetyl glucosamine | 16.0 | 8.4 | 3.0 | 1.6 | 29.5 | 28.7 | 27.9 | 45.2 |
| N-Acetyl mannosamine | 4.1 | 2.2 | 0.0 | 0.0 | 2.2 | 2.1 | 0.7 | 1.2 |
| Σ= | 191.3 | 179.9 | 102.8 | 61.8 | ||||
Mass is expressed in μg and weight % is relative to the total carbohydrate.