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. Author manuscript; available in PMC: 2014 Nov 24.
Published in final edited form as: Clin Cancer Res. 2010 Jul 20;16(15):4057–4065. doi: 10.1158/1078-0432.CCR-10-1093

Figure 1. Representative gp100209-217 HLA-*A201-restricted tetramer analysis on CD8+ T cells following mouse gp100 DNA vaccination.

Figure 1

Gp100209-217 and gp100280-288 tetramer staining was performed at baseline, week 18 and week 30, and analyzed via multi-parameter flow cytometry. Tetramer positive cells, defined as greater than 3 times the standard deviation above the mean baseline value and an absolute value above 0.1%, were gated on CD3+CD8+ T cells. The above representative dot-plots demonstrate this gating within a positive patient's CD3+ lymphocyte population.