Skip to main content
. Author manuscript; available in PMC: 2015 Sep 18.
Published in final edited form as: Immunity. 2014 Sep 18;41(3):493–502. doi: 10.1016/j.immuni.2014.08.014

Figure 1. Memory CD4+ T cells and myeloid cells express SAMHD1.

Figure 1

SAMHD1 mRNA in naïve CD4+ T cells, CD28+ memory CD4+ T cells, CD28− memory CD4+ T cells, and myeloid cells in peripheral blood of SIV-uninfected (A) and SIV-infected (B) rhesus macaques. Expression relative to β-actin mRNA. (C) Total and phosphorylated SAMHD1 protein in naïve CD4+ T cells, CD28+ memory CD4+ T cells, CD28− memory CD4+ T cells, and myeloid cells in peripheral blood of SIV-uninfected animals. Forty μg of primary cell extract or 20 μg of THP-1 cell extract were separated by SDS-PAGE and Western blotted using antibodies against SAMHD1, phosphorylated SAMHD1 or β-actin. Horizontal lines indicate the median. Western blots are representative of three experiments.