Figure 1.

Determination of microvascular density and vascular architecture (a, b) and VEGF immunohistochemistry (c, d). CD34-stained fields (1.0 mm²) (a) are input into the image analyzer, and each vessel contour is manually traced in order to measure vessel density (number), vessel area (%), vessel diameter (μm), vessel perimeter (μm), and vessel roundness (0-1) (b). The area of the black box in (a) is magnified to (b). VEGF immunohistochemistry: positive tumor with clear cytoplasmic staining (c) and negative tumor (d). Original magnification (a): 200x, (c), (d): 400x. Bar 100 μm.