. 2014 Apr 1;7(4):332–346. doi: 10.1111/1751-7915.12125

Table 1.

Primers used in the present study

Analysis	Primers/probes	Sequences (5'–3’)	Target	Ta (⁰C)	Amplicon size (bp)	Reference
DGGE	344F	ACGGGYGCAGCAGGCGCGA	Archaea	56	191	Yu and colleagues (2008)
	GC-519R^a	ATTACCGCGGCKGCTG	Archaea	56	191	Yu and colleagues (2008)
	GC-357F^a	CCTACGGGAGGCAGCAG	Bacteria	56	194	Yu and Morrison (2004b)
	519R	ATTACCGCGGCKGCTG	Bacteria	56	194	Yu and Morrison (2004b)
Pyrosequencing	ArcF-A^b	WCYGGTTGATCCYGCCRG	Archaea	56	534	Nelson (2011)
	ArcR-B^c	YGGTRTTACCGCGGCGGCT	Archaea	56	534	Nelson (2011)
	BactF-A^b	AKRGTTYGATYNTGGCTCAG	Bacteria	56	532	Nelson (2011)
	BactR-B^c	GTNTBACCGCDGCTGCTG	Bacteria	56	532	Nelson (2011)
qPCR	Mbt-202F	CGCCTAAGGATGGATC	Methanobacterium	60	148
	Mbt-341Taq^d	FAM-CGCGAAACCTCCGCAATGC-BHQ				Nelson (2011)
	Mbt-399R	TAAGAGTGGCACTTGGGK
	Mcu-934F	AGGAATTGGCGGGGGAGCAC	Methanoculleus	60	309	Franke-Whittle and colleagues (2009); Shigematsu and colleagues (2003)
	Mcu-1023Taq^d	Cy5-GAATGATTGCCGGGCTGAAGACTC-BHQ
	Mcu-1200R	CCGGATAATTCGGGGCATGCTG
	MB1b	CGGTTTGGTCAGTCCTCCGG	Methanosarcina	60	271	Shigematsu and colleagues (2003)
	SAR761Taq^d	HEX-ACCAGAACGGGTTCGACGGTGAGG-BHQ
	SAR835R	AGACACGGTCGCGCCATGCCT
	MS1b	CCGGCCGGATAAGTCTCTTGA	Methanosaeta	60	272	Shigematsu and colleagues (2003)
	SAE761Taq^d	FAM-ACCAGAACGGACCTGACGGCAAGG-BHQ
	SAE835R	GACAACGGTCGCACCGTGGCC
	Mcp193F	TCCTCGAAAGATCCGTC	Methanocorpusculaceae	60	314	Goberna and colleagues (2010)
	Mcp491R	GCCYTGCCCTTTCTTCAC	Methanocorpusculaceae	60	314	This study

^a.

A 40 nt GC clamp was attached at the 5’ end of the primer GC-519R and GC-357F.

^b.

A 21 nt adaptor A and TCAG key was attached at the 5’ end.

^c.

A 25 nt adaptor B and TCAG key and a 10 nt sample-specific barcode sequence was attached at the 5’ end.

^d.

The probes contain a fluorescence dye at 5’ end and a quencher BHQ at 3’.