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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: J Immunol. 2014 Jul 7;193(4):1709–1716. doi: 10.4049/jimmunol.1400034

Fig 6. NK cells negatively regulate CD8 T cell expansion after LD IL2 and anti-TGFβ stimulation in a Fas-FasL dependent manner to reduce CD8 T cell dependent toxicity.

Fig 6

Single cell-suspension from spleens and LN were stained as previously described to determine T cell distribution at 24 and 72h. (A) Total number of CD4, CD8 T cells, and Tregs 24h after end of treatment from spleens (B) Percentage of effector and naive CD8 T cells 24h post-treatment. (C–D) Total number of effector and bystander CD8 T cells for the spleen and LN at 24h post-treatment. (E) CD8 T cell-specific tumor lysis. (F–G) ALT and IL6 serum levels at 24h. (H) MFI expression of Fas for naive, effector and bystander CD8 T cells is shown. WT or FasL-deficient mice were treated as previously and spleens were collected 24h after treatment. (I–J) Fold increase of NK and CD8 T cells 24h after treatment in WT and FasL-deficient mice. (K) Fold change in MFI expression of Fas for effector CD8 T cells in WT and FasL-deficient mice. Data are representative of 2–5 independent experiments (with 3 mice per group (mean ± SEM). One-Way Anova or Two-Way Anova was used to assess significance (n.s: no significance, *p<0.05, **p<0.01, ***p<0.001).