Figure 1.

Antigen-specific circulating T cell responses following sipuleucel-T treatment. T cell proliferation to PA2024 (A) and PAP (B) were measured by ³H-thymidine incorporation in cultured PBMC. IFNγ responses to PA2024 (C) and prostatic acid phosphatase (D) were measured by enzyme-linked immunospot (ELISPOT) in cultured peripheral blood mononuclear cells. Each measure is shown at baseline, pre–radical prostatectomy (RP) (following sipuleucel-T infusions), six weeks post-RP, and 12 weeks post-RP. Horizontal bars represent the median values of patients with all four timepoints available. * Denotes two-sided P under .05, Wilcoxon matched pairs test for comparison with baseline. PA2024 = recombinant fusion protein; PAP = prostatic acid phosphatase; RP = radical prostatectomy.