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. 2014 Sep 17;18(3):267–275. doi: 10.5717/jenb.2014.18.3.267

Table 3.

Nucleotide sequence of primer in TNF-α, IL-6 and GAPDH mRNA

sequence TM (C°)
TNF-α (+) 5’- TCC AGA CTT CCT TGA GAC ACG -3’ 60
(-) 5’- CCC GGT CTC CCA AAT AAA TAC -3’ 59
IL-6 (+) 5’- GAT GAG TAC AAA AGT CCT GAT CCA -3’ 59
(-) 5’- CTG CAG CCA CTG GTT CTG T -3’ 60
GAPDH (+) 5’- CAA CGA CCA CTT TGT CAA GC -3’ 59
(-) 5’- GGT GGT CCA GGG GTC TTA CT -3’ 60