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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Immunol. 2014 Sep 22;193(9):4457–4468. doi: 10.4049/jimmunol.1401125

Figure 1. Cytokine production by plate-bound anti-CD3e stimulated splenocytes from control and HG mice.

Figure 1

Media from cultures of splenocytes isolated from control or HG mice was collected after 24, 48 and 72 h of plate-bound anti-CD3e stimulation. A, Pooled samples from 3 individual cultures were analyzed for Th1 cytokines IFN-γ, TNF-α, IL-12p40 and IL-2 by multiplex ELISA. B, Pooled samples from 3 individual cultures were analyzed for Th2 cytokines IL-10 and IL-13 by multiplex ELISA. C, Pooled samples from 3 individual cultures were analyzed for Th17 cytokines IL-17 and GMCSF by multiplex ELISA. D, IFN-γ, IL-10 and IL-17 production were analyzed in media from individual cell cultures by ELISA. Line represents the mean of the values (n=5). E, Representative histogram of IFN-γ staining (left), percentage of IFN-γ+ cells (center) and mean fluorescent intensity (MFI) (right) in CD4+ T cells stimulated with plate-bound anti-CD3e (2.5 µg/ml) for 48 h. Statistical differences were analyzed by Student’s t test for each time point, *p<0.05, **p<0.01.