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Fluorescence lifetime is more precise than fluorescence intensity. Intensity (black squares) and lifetime (red triangles) of rhodamine B (1 μM in water) were calculated from waveforms acquired in 192 wells of a 384-well microplate. Lifetime CV is 0.7% (coefficient of variation = SD/mean), 5-fold more precise than intensity CV (3.5%) for this short lifetime dye. Left axis is total intensity divided by mean and right axis is centered and scaled in proportion.
